Jump to main content
Jump to site search


Tuning of resonance energy transfer from 4',6-diamidino-2-phenylindole to ultrasmall silver nanocluster across lipid bilayer

Abstract

Here we have developed a simple liposome-based donor-acceptor system across the lipid bilayer using 4',6-diamidino-2-phenylindole (DAPI) as donor and ultasmall ligand-capped silver nanocluster (Ag NC) as acceptor. The process of Förster resonance energy transfer (FRET) between DAPI and Ag NC across the liposome bilayer has been demonstrated using steady-state and time-resolved fluorescence spectroscopy. The synthesized Ag NCs with majority of Ag4 and Ag5 core have been characterized using FTIR, mass spectrometry, HRTEM, UV-Vis and PL spectroscopy. Scanning electron microscopy (SEM) and dynamic light scattering (DLS) measurements reveal that the synthesized liposomes are small unilamilar vesicles (SUV) with mean hydrodynamic diameter of 86.91 ± 6.41 nm. By using two distinct synthetic methods, we have able to identify two selective binding sites of DAPI with liposome namely, the liposome surface and hydrophilic aqueous core. Fluorescence confocal microscopy confirms the location of donor DAPI within different locations of liposome. It has been observed that association of DAPI at the surface of liposome results in less efficient energy transfer to Ag NC compared to that in bulk aqueous medium. Energy transfer efficiency decreases from a value of 0.76 in bulk aqueous medium to a value of 0.39 for surface-associated DAPI. On the other hand encapsulation of DAPI into the hydrophilic aqueous core of liposome results in complete inhibition of the FRET process as a consequence of increased separation distance beyound the FRET range. Hence, our study illustrates that the present DAPI-Ag NC pair can be use as a FRET marker to explore various fundamental processes accross the cell membrane.

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Aug 2017, accepted on 14 Sep 2017 and first published on 14 Sep 2017


Article type: Paper
DOI: 10.1039/C7CP05225A
Citation: Phys. Chem. Chem. Phys., 2017, Accepted Manuscript
  •   Request permissions

    Tuning of resonance energy transfer from 4',6-diamidino-2-phenylindole to ultrasmall silver nanocluster across lipid bilayer

    J. K. Vaishnav and T. K. Mukherjee, Phys. Chem. Chem. Phys., 2017, Accepted Manuscript , DOI: 10.1039/C7CP05225A

Search articles by author

Spotlight

Advertisements