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The first successful observation of in-cell NMR signals of DNA and RNA in living human cells

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Abstract

In order to understand intracellular biological events, information on the structure, dynamics and interaction of proteins and nucleic acids in living cells is of crucial importance. In-cell NMR is a promising method to obtain this information. Although NMR signals of proteins in human cells have been reported, those of nucleic acids were reported only in Xenopus laevis oocytes, i.e., not in human cells. Here, DNA and RNA were introduced into human cells by means of pore formation by bacterial toxin streptolysin O and subsequent resealing. Then, NMR signals of DNA and RNA were successfully observed for the first time in living human cells. The observed signals directly suggested the formation of DNA and RNA hairpin structures in living human cells.

Graphical abstract: The first successful observation of in-cell NMR signals of DNA and RNA in living human cells

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Publication details

The article was received on 31 Jul 2017, accepted on 27 Sep 2017 and first published on 27 Sep 2017


Article type: Communication
DOI: 10.1039/C7CP05188C
Citation: Phys. Chem. Chem. Phys., 2017, Advance Article
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    The first successful observation of in-cell NMR signals of DNA and RNA in living human cells

    Y. Yamaoki, A. Kiyoishi, M. Miyake, F. Kano, M. Murata, T. Nagata and M. Katahira, Phys. Chem. Chem. Phys., 2017, Advance Article , DOI: 10.1039/C7CP05188C

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