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Issue 44, 2017
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Enzymatic degradation of dimensionally constrained polyhydroxybutyrate films

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Abstract

The effect of dimensional constraint, imparted by a variation in film thickness, on the enzymatic degradation of polyhydroxybutyrate (PHB) is reported. The characterization of the crystalline structure and the surface topography of solvent-cast PHB thin films revealed strong correlations between film thickness and both crystallinity and crystal anisotropy, with the polymer film becoming more amorphous with decreasing thickness. The enzymatic degradation of the PHB films was characterized using a high precision diffraction metrology, which enabled the visualization of small variations in the degradation behavior. The results show that the degradation rate increases with decreasing thickness due to the corresponding decrease in crystallinity. However, in a nanoscopic ultra-thin PHB specimen, produced by μ-transfer molding, enzymatic degradation was impeded. The enzymatic degradation rate of the PHB films therefore was found to exhibit a discontinuous trend with respect to film thickness: initially increasing as film thickness was reduced, and then decreasing dramatically once the thickness was reduced to tens of nanometers. In this regime, enzymatic degradation was hindered by the absence of crystalline regions in the films. These results show that a nano-dimensional constraint on PHB films can result in specimens with a tunable response to extracellular enzymes.

Graphical abstract: Enzymatic degradation of dimensionally constrained polyhydroxybutyrate films

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Publication details

The article was received on 28 Jul 2017, accepted on 12 Oct 2017 and first published on 12 Oct 2017


Article type: Paper
DOI: 10.1039/C7CP05133F
Citation: Phys. Chem. Chem. Phys., 2017,19, 30021-30030
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    Enzymatic degradation of dimensionally constrained polyhydroxybutyrate films

    P. Anbukarasu, D. Sauvageau and A. L. Elias, Phys. Chem. Chem. Phys., 2017, 19, 30021
    DOI: 10.1039/C7CP05133F

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