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Issue 37, 2017
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Changes in dynamics of α-chymotrypsin due to covalent inhibitors investigated by elastic incoherent neutron scattering

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Abstract

An essential role of enzymes is to catalyze various chemical reactions in the human body and inhibition of the enzymatic activity by small molecules is the mechanism of action of many drugs or tool compounds used to study biological processes. Here, we investigate the effect on the dynamics of the serine protease α-chymotrypsin when in complex with two different covalently bound inhibitors using elastic incoherent neutron scattering. The results show that the inhibited enzyme displays enhanced dynamics compared to the free form. The difference was prominent at higher temperatures (240–310 K) and the type of motions that differ include both small amplitude motions, such as hydrogen atom rotations around a methyl group, and large amplitude motions, such as amino acid side chain movements. The measurements were analyzed with multivariate methods in addition to the standard univariate methods, allowing for a more in-depth analysis of the types of motions that differ between the two forms. The binding strength of an inhibitor is linked to the changes in dynamics occurring during the inhibitor-enzyme binding event and thus these results may aid in the deconvolution of this fundamental event and in the design of new inhibitors.

Graphical abstract: Changes in dynamics of α-chymotrypsin due to covalent inhibitors investigated by elastic incoherent neutron scattering

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Publication details

The article was received on 15 Jun 2017, accepted on 01 Sep 2017 and first published on 04 Sep 2017


Article type: Paper
DOI: 10.1039/C7CP04041E
Citation: Phys. Chem. Chem. Phys., 2017,19, 25369-25379
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    Changes in dynamics of α-chymotrypsin due to covalent inhibitors investigated by elastic incoherent neutron scattering

    C. D. Andersson, N. Martinez, D. Zeller, S. H. Rondahl, M. M. Koza, B. Frick, F. Ekström, J. Peters and A. Linusson, Phys. Chem. Chem. Phys., 2017, 19, 25369
    DOI: 10.1039/C7CP04041E

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