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Lysine-based amino-functionalized lipids for gene transfection: the protonation state in monolayers at the air–liquid interface

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Abstract

Cationic lipids are considered as non-viral carriers for genetic material used in gene therapy. They have no carcinogenic potential and cause low immune response compared to existing viral systems. The protonation degree of these cationic lipids is a crucial parameter for the binding behavior of polynucleotides (e.g., DNA). Newly synthesized peptide-mimic lysine-based amino-functionalized lipids have been investigated in 2D models as monolayers at the air–liquid interface. Standard surface pressure – area isotherms have been measured to prove the layer stability. Total reflection X-ray fluorescence (TRXF) has been used as a surface sensitive analytical method to estimate the amount of counterions at the head groups. Using a standard sample as a reference, the protonation degree of these cationic lipids can be quantified on buffers with different pH values. It is found that the protonation degree depends linearly on the packing density of the lipid monolayer.

Graphical abstract: Lysine-based amino-functionalized lipids for gene transfection: the protonation state in monolayers at the air–liquid interface

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Publication details

The article was received on 10 May 2017, accepted on 09 Jul 2017 and first published on 11 Jul 2017


Article type: Paper
DOI: 10.1039/C7CP03107F
Citation: Phys. Chem. Chem. Phys., 2017, Advance Article
  • Open access: Creative Commons BY license
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    Lysine-based amino-functionalized lipids for gene transfection: the protonation state in monolayers at the air–liquid interface

    S. Tassler, C. Wölk, C. Janich, B. Dobner and G. Brezesinski, Phys. Chem. Chem. Phys., 2017, Advance Article , DOI: 10.1039/C7CP03107F

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