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Issue 26, 2017
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Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms

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Abstract

The interaction of the porphyrin derivative H2TCPPSpm4, having spermine pendants in the four meso positions, with the G-quadruplex (GQ) structure formed by the DNA aptamer TGGGAG has been investigated by means of UV, electronic circular dichroism and PAGE studies. The results reported here demonstrate that the porphyrin derivative is capable of stabilizing or destabilizing the higher-ordered structures of parallel GQs, depending on the method used to reach their relative stoichiometry (titration vs. single addition). Noteworthily, when two equivalents of H2TCPPSpm4 were mixed directly with one equivalent of the (TGGGAG)4 GQ to reach a 2 : 1 H2TCPPSpm4 : GQ ratio T1/2 higher than 80 °C was also observed confirming the presence of higher-ordered GQ structures.

Graphical abstract: Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms

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Publication details

The article was received on 30 Apr 2017, accepted on 09 Jun 2017 and first published on 12 Jun 2017


Article type: Paper
DOI: 10.1039/C7CP02816D
Citation: Phys. Chem. Chem. Phys., 2017,19, 17404-17410
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    Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms

    A. D'Urso, R. Randazzo, V. Rizzo, C. M. A. Gangemi, V. Romanucci, A. Zarrelli, G. Tomaselli, D. Milardi, N. Borbone, R. Purrello, G. Piccialli, G. Di Fabio and G. Oliviero, Phys. Chem. Chem. Phys., 2017, 19, 17404
    DOI: 10.1039/C7CP02816D

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