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Issue 35, 2017
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Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

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Abstract

Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42–208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide and peptidoglycan layers, direct membrane disruption of both Gram-negative and Gram-positive bacteria is suppressed. However, LDH nanoparticles cause size-dependent charge reversal and resulting flocculation of both liposomes and bacteria, which may provide a mechanism for bacterial confinement or clearance. Taken together, these findings demonstrate a set of previously unknown behaviors, including synergistic membrane destabilization and dual confinement/killing of bacteria through combined LDH/AMP exposure, of potential therapeutic interest.

Graphical abstract: Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

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Publication details

The article was received on 25 Apr 2017, accepted on 28 Jun 2017 and first published on 06 Jul 2017


Article type: Paper
DOI: 10.1039/C7CP02701J
Citation: Phys. Chem. Chem. Phys., 2017,19, 23832-23842
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    Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

    S. Malekkhaiat Häffner, L. Nyström, R. Nordström, Z. P. Xu, M. Davoudi, A. Schmidtchen and M. Malmsten, Phys. Chem. Chem. Phys., 2017, 19, 23832
    DOI: 10.1039/C7CP02701J

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