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Issue 20, 2017
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Physical chemistry in a single live cell: confocal microscopy

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Abstract

A live cell is a complex, yet extremely important container. Understanding the dynamics in a selected intracellular component is a challenging task. We have recently made significant progress in this direction using a confocal microscope as a tool. The smallest size of the focused spot in a confocal microscope is ∼0.2 μm (200 nm). This is nearly one hundred times smaller than the size of a live cell. Thus, one can selectively study different intracellular components/organelles in a live cell. In this paper, we discuss how one can image different intracellular components/organelles, record fluorescence spectra and decay at different locations, ascertain local polarity and viscosity, and monitor the dynamics of solvation, proton transfer, red-ox and other phenomena at specified locations/organelles inside a cell. We will highlight how this knowledge enriched us in differentiating between cancer and non-cancer cells, 3D tumor spheroids and towards drug delivery.

Graphical abstract: Physical chemistry in a single live cell: confocal microscopy

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Publication details

The article was received on 06 Apr 2017, accepted on 25 Apr 2017 and first published on 26 Apr 2017


Article type: Perspective
DOI: 10.1039/C7CP02228J
Citation: Phys. Chem. Chem. Phys., 2017,19, 12620-12627
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    Physical chemistry in a single live cell: confocal microscopy

    Md. A. Amin, S. Nandi, P. Mondal, T. Mahata, S. Ghosh and K. Bhattacharyya, Phys. Chem. Chem. Phys., 2017, 19, 12620
    DOI: 10.1039/C7CP02228J

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