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The Mechanism of the Ser-(cis)Ser-Lys Catalytic Triad of Peptide Amidases.

Abstract

In this paper, we report a theoretical investigation about the catalytic mechanism of peptide amidases that involve a Ser-(cis)Ser-Lys catalytic triad. Previous suggestions propose that the catalytic mechanism of these enzymes should be different from the one present in the classic Ser-His-Asp catalytic triad. The theoretical and computational results obtained in this work indicate the opposite idea, showing that both mechanisms are very similar and only few differences are observed between both reactions. The results reveal that different alignment of the Ser-(cis)Ser-Lys catalytic triad in relation to the classical Ser-His-Asp triad provide a better stabilisation of the reaction intermediates, and therefore turn these enzymes catalytically more efficient. The catalytic mechanism has been determined at the M06-2X/6-311++G**//B3LYP/6-31G* level of theory and requires five sequential steps instead of the two that are generally proposed: (i) nucleophilic attack of serine to the carbonyl group of the substrate, forming the first tetrahedral intermediate, (ii) formation of the acyl–enzyme complex, ii) release of ammonia product , (iv) nucleophilic attack of a water molecule forming the second tetrahedral intermediate, and (iv) the release of the product of the reaction, the carboxylic acid. The computational results suggest that the rate-limiting step if the first one that requires an activation free energy of 15.93 kcal/mol. This result agrees very well with the available experimental data that predicts a reaction rate of 2200 s-1 which, corresponds to a free energy barrier of 14 kcal/mol.

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Publication details

The article was received on 13 Jan 2017, accepted on 28 Mar 2017 and first published on 30 Mar 2017


Article type: Paper
DOI: 10.1039/C7CP00277G
Citation: Phys. Chem. Chem. Phys., 2017, Accepted Manuscript
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    The Mechanism of the Ser-(cis)Ser-Lys Catalytic Triad of Peptide Amidases.

    N. M. F. S. A. S. Cerqueira, N. S. H. N. Moorthy, P. A. Fernandes and M. J. Ramos, Phys. Chem. Chem. Phys., 2017, Accepted Manuscript , DOI: 10.1039/C7CP00277G

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