Jump to main content
Jump to site search


A Sensitive Fluorescence Probe for the Polar Solvation Dynamics at Protein- Surfactant Interface

Abstract

Relaxation dynamics at the surface of biologically important macromolecules is important taking into account of their functionality in molecular recognition. Over the years it has been shown that solvation dynamics of a fluorescence probe at biomolecular sufaces and interfaces account for the relaxation dynamics of polar residues and associated water molecules. However, the sensitivity of the dynamics depends largely on the localization and exposure of the probe. For noncovalent fluorescence probes, localization at the region of interest in addition to the surface exposure, is an added challenge compared to the covalently attached probes at the biological interfaces. Here we have used a synthesized donor-acceptor type dipolar fluorophore, 6-acetyl-(2-((4-hydroxycyclohexyl) (methyl) amino) naphthalene (ACYMAN) for the investigation of solvation dynamics of a model protein-surfactant interface. A significant structural rearrangement of a model Histone protein (H1) upon interaction with anionic surfactant sodium dodecyl sulphate (SDS) as revealed from the circular dichroism (CD) studies is nicely corroborated in the solvation dynamics of the probe at the interface. The polarization gated fluorescence anisotropy of the probe compared to that at the SDS micellar surface clearly reveals the localization of the probe at the protein-surfactant interface. We have also compared the sensitivity of ACYMAN with other solvation probes including Coumarin 500 (C500) and 4-(dicyanomethylene)-2-methyl-6-(p-dimethylamino-styryl) 4H-pyran (DCM). In comparison to ACYMAN, both C500 and DCM fail to probe the interfacial solvation dynamics of a model protein-surfactant interface. While C500 is found to be delocalized from the protein-surfactant interface, DCM becomes destabilized upon the formation of the interface (protein-surfactant complex). The time scales obtained from this novel probe have also been compared with other femtosecond resolved studies and molecular dynamics simulation.

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 24 Dec 2016, accepted on 18 Apr 2017 and first published on 18 Apr 2017


Article type: Paper
DOI: 10.1039/C6CP08804J
Citation: Phys. Chem. Chem. Phys., 2017, Accepted Manuscript
  •   Request permissions

    A Sensitive Fluorescence Probe for the Polar Solvation Dynamics at Protein- Surfactant Interface

    P. Singh, S. Choudhury, S. Singha, Y. W. Jun, S. Chakraborty, J. Sengupta, R. DAS, K. H. Ahn and S. K. Pal, Phys. Chem. Chem. Phys., 2017, Accepted Manuscript , DOI: 10.1039/C6CP08804J

Search articles by author