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Issue 17, 2017
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Switching from adduct formation to electron transfer in a light–oxygen–voltage domain containing the reactive cysteine

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Abstract

LOV (light-, oxygen- or voltage-sensitive) domains act as photosensory units of many prokaryotic and eukaryotic proteins. Upon blue light excitation they undergo a photocycle via the excited triplet state of their flavin chromophore yielding the flavin–cysteinyl adduct. Adduct formation is highly conserved among all LOV domains and constitutes the primary step of LOV domain signaling. But recently, it has been shown that signal propagation can also be triggered by flavin photoreduction to the neutral semiquinone offering new prospects for protein engineering. This, however, requires mutation of the photo-active Cys. Here, we report on LOV1 mutants of C. reinhardtii phototropin in which adduct formation is suppressed although the photo-active Cys is present. Introduction of a Tyr into the LOV core induces a proton coupled electron transfer towards the flavin chromophore. Flavin radical species are formed via either the excited flavin singlet or triplet state depending on the geometry of donor and acceptor. This photoreductive pathway resembles the photoreaction observed in other blue light photoreceptors, e.g. blue-light sensors using flavin adenine dinucleotide (BLUF) domains or cryptochromes. The ability to tune the photoreactivity of the flavin chromophore inside the LOV core has implications for the mechanism of adduct formation in the wild type and may be of use for protein engineering.

Graphical abstract: Switching from adduct formation to electron transfer in a light–oxygen–voltage domain containing the reactive cysteine

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Publication details

The article was received on 07 Dec 2016, accepted on 13 Feb 2017 and first published on 20 Feb 2017


Article type: Paper
DOI: 10.1039/C6CP08370F
Citation: Phys. Chem. Chem. Phys., 2017,19, 10808-10819
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    Switching from adduct formation to electron transfer in a light–oxygen–voltage domain containing the reactive cysteine

    K. Magerl, I. Stambolic and B. Dick, Phys. Chem. Chem. Phys., 2017, 19, 10808
    DOI: 10.1039/C6CP08370F

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