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Conformational dynamics and self-association of intrinsically disordered Huntingtin exon 1 in cells

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Abstract

Huntington's disease is caused by a CAG trinucleotide expansion mutation in the Huntingtin gene that leads to an artificially long polyglutamine sequence in the Huntingtin protein. A key feature of the disease is the intracellular aggregation of the Huntingtin exon 1 protein (Httex1) into micrometer sized inclusion bodies. The aggregation process of Httex1 has been extensively studied in vitro, however, the crucial early events of nucleation and aggregation in the cell remain elusive. Here, we studied the conformational dynamics and self-association of Httex1 by in-cell experiments using laser-induced temperature jumps and analytical ultracentrifugation. Both short and long polyglutamine variants of Httex1 underwent an apparent temperature-induced conformational collapse. The temperature jumps generated a population of kinetically trapped species selectively for the longer polyglutamine variants of Httex1 proteins. Their occurrence correlated with the formation of inclusion bodies suggesting that such species trigger further self-association.

Graphical abstract: Conformational dynamics and self-association of intrinsically disordered Huntingtin exon 1 in cells

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Publication details

The article was received on 29 Nov 2016, accepted on 09 Jan 2017, published on 09 Jan 2017 and first published online on 09 Jan 2017


Article type: Paper
DOI: 10.1039/C6CP08167C
Citation: Phys. Chem. Chem. Phys., 2017, Advance Article
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    Conformational dynamics and self-association of intrinsically disordered Huntingtin exon 1 in cells

    S. Büning, A. Sharma, S. Vachharajani, E. Newcombe, A. Ormsby, M. Gao, D. Gnutt, T. Vöpel, D. M. Hatters and S. Ebbinghaus, Phys. Chem. Chem. Phys., 2017, Advance Article , DOI: 10.1039/C6CP08167C

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