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Issue 24, 2017
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Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent

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Abstract

We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a “common dispersing agent”. The common dispersing agent is used to dilute the crystal seeds. Seven proteins were screened, and more crystallization conditions were found. X-ray diffraction showed that the combined method is practical and useful for obtaining high-quality protein crystals.

Graphical abstract: Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent

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Publication details

The article was received on 06 Apr 2017, accepted on 31 May 2017 and first published on 31 May 2017


Article type: Communication
DOI: 10.1039/C7CE00664K
Citation: CrystEngComm, 2017,19, 3237-3243
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    Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent

    H. Hou, B. Wang, P. Xie, Y. Guo, J. Li and D. Yin, CrystEngComm, 2017, 19, 3237
    DOI: 10.1039/C7CE00664K

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