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Microfluidic formation of proteinosomes

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Abstract

Herein we describe a novel microfluidic method for the generation of proteinosome micro-droplets, based on bovine serum albumin and glucose oxidase conjugated to PNIPAAm chains. The size of such water-in-oil droplets is regulated via control of the input reagent flow rate, with generated proteinosome populations exhibiting narrower size distributions than those observed when using standard bulk methodologies. Importantly, proteinosomes transferred from an oil to an aqueous-environment remain intact, become fully hydrated and exhibit an increase in average size. Moreover, functional proteinosomes prepared via microfluidics exhibit lower Km values and higher enzymatic activities than proteinosomes produced by bulk methodologies.

Graphical abstract: Microfluidic formation of proteinosomes

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Publication details

The article was received on 03 Nov 2017, accepted on 06 Dec 2017 and first published on 06 Dec 2017


Article type: Communication
DOI: 10.1039/C7CC08466H
Citation: Chem. Commun., 2018, Advance Article
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    Microfluidic formation of proteinosomes

    M. Ugrinic, A. Zambrano, S. Berger, S. Mann, T.-Y. D. Tang and A. deMello, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C7CC08466H

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