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Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

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Abstract

We found that Escherichia coli MazF toxin, an ACA-sequence-specific endoribonuclease, was sensitive to N6-methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Furthermore, the approach could be used for inhibitor screening.

Graphical abstract: Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

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Publication details

The article was received on 04 Oct 2017, accepted on 12 Nov 2017 and first published on 13 Nov 2017


Article type: Communication
DOI: 10.1039/C7CC07699A
Citation: Chem. Commun., 2017, Advance Article
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    Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

    M. Imanishi, S. Tsuji, A. Suda and S. Futaki, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC07699A

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