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A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging

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Abstract

The far-red emissive fluorescent probe CaPF-1 based on a phospha-fluorescein scaffold enables the detection of cytosolic calcium ions in living cells. The probe can be excited in the red region (λabs = 636 nm) and exhibits a sufficiently high fluorescence turn-on response in the far-red region (λem = 663 nm) upon complexation with calcium ions. The hydrophilic and anionic characteristics of this phospha-fluorescein fluorophore allowed the cytosolic localization of CaPF-1. Moreover, it was possible to visualize histamine-induced calcium oscillation in HeLa cells using CaPF-1.

Graphical abstract: A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging

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Publication details

The article was received on 20 Sep 2017, accepted on 06 Dec 2017 and first published on 07 Dec 2017


Article type: Communication
DOI: 10.1039/C7CC07344E
Citation: Chem. Commun., 2018, Advance Article
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    A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging

    H. Ogasawara, M. Grzybowski, R. Hosokawa, Y. Sato, M. Taki and S. Yamaguchi, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C7CC07344E

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