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Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells

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Abstract

Endogenous protein labeling is one of the most invaluable methods for studying the bona fide functions of proteins in live cells. However, multi-molecular crowding conditions, such as those that occur in live cells, hamper the highly selective chemical labeling of a protein of interest (POI). We herein describe how the efficient coupling of molecular recognition with a chemical reaction is crucial for selective protein labeling. Recognition-driven protein labeling is carried out by a synthetic labeling reagent containing a protein (recognition) ligand, a reporter tag, and a reactive moiety. The molecular recognition of a POI can be used to greatly enhance the reaction kinetics and protein selectivity, even under live cell conditions. In this review, we also briefly discuss how such selective chemical labeling of an endogenous protein can have a variety of applications at the interface of chemistry and biology.

Graphical abstract: Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells

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Publication details

The article was received on 13 Sep 2017, accepted on 05 Oct 2017 and first published on 13 Oct 2017


Article type: Feature Article
DOI: 10.1039/C7CC07177A
Citation: Chem. Commun., 2017, Advance Article
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    Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells

    K. Amaike, T. Tamura and I. Hamachi, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC07177A

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