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Two-photon excitable and ratiometric fluorogenic nitric oxide photoreleaser and its biological applications

Abstract

Nitric oxide (NO) photoreleasers allowing ratiometric imaging or visualization of NO release in situ have currently not been realized. We report for the first time the development of a two-photon excitable NO photoreleaser, CNNO, for ratiometric imaging and tracking of NO release in live cells. Upon one-photon or two-photon irradiation, CNNO displays high photosensitivity, rapid release, and satisfied stability in physiological buffer solution and cell culture. Importantly, the NO release is accompanied by dramatic fluorescence ratiometric variation. CNNO exhibits the merits of spatiotemporal controlling in both the site-specific NO release in the selected cell culture region and the controllable vasodilation of mouse aorta ex vivo. Therefore, CNNO may provide a valuable tool for not only NO storing, but the fundamental interrogations of NO-mediated biology with precise spatiotemporal control.

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Publication details

The article was received on 30 Aug 2017, accepted on 12 Oct 2017 and first published on 12 Oct 2017


Article type: Communication
DOI: 10.1039/C7CC06820D
Citation: Chem. Commun., 2017, Accepted Manuscript
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    Two-photon excitable and ratiometric fluorogenic nitric oxide photoreleaser and its biological applications

    X. Xie, J. Fan, M. Liang, Y. Li, X. Jiao, X. Wang and B. Tang, Chem. Commun., 2017, Accepted Manuscript , DOI: 10.1039/C7CC06820D

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