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Mapping platinum adducts on yeast ribosomal RNA using high-throughput sequencing

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Abstract

Methods to map small-molecule binding sites on cellular RNAs are important for understanding interactions with both endogenous and exogenous compounds. Pt(II) reagents are well-known DNA and RNA crosslinking agents, but sequence-specific and genome-wide identification of Pt targets following in-cell treatment is challenging. Here we describe application of high-throughput ‘Pt-Seq’ to identify Pt-rRNA adducts following treatment of S. cerevisiae with cisplatin.

Graphical abstract: Mapping platinum adducts on yeast ribosomal RNA using high-throughput sequencing

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Publication details

The article was received on 12 Sep 2017, accepted on 24 Oct 2017 and first published on 27 Oct 2017


Article type: Communication
DOI: 10.1039/C7CC06708A
Citation: Chem. Commun., 2017, Advance Article
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    Mapping platinum adducts on yeast ribosomal RNA using high-throughput sequencing

    K. Plakos and V. J. DeRose, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC06708A

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