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Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

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Abstract

The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2′,5′-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.

Graphical abstract: Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

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Publication details

The article was received on 26 Aug 2017, accepted on 29 Sep 2017 and first published on 29 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC06703H
Citation: Chem. Commun., 2017, Advance Article
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    Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

    T. J. Carrocci, L. Lohe, M. J. Ashton, C. Höbartner and A. A. Hoskins, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC06703H

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