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A novel method to control carryover contamination in isothermal nucleic acid amplification

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Abstract

We developed a novel method to control carryover contamination in loop-mediated isothermal amplification (LAMP) by primer engineering to carry recognition sites for a restriction endonuclease, providing a robust ability to eliminate carryover contaminants.

Graphical abstract: A novel method to control carryover contamination in isothermal nucleic acid amplification

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Publication details

The article was received on 17 Aug 2017, accepted on 07 Sep 2017 and first published on 07 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC06469A
Citation: Chem. Commun., 2017, Advance Article
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    A novel method to control carryover contamination in isothermal nucleic acid amplification

    C. Ma, F. Wang, X. Wang, L. Han, H. Jing, H. Zhang and C. Shi, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC06469A

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