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Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

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Abstract

A label-free and general thermo-controlled split apta-PCR strategy was first developed for the sensitive and specific detection of cancer cells. By integrating the temperature-responsive function of split aptamers with PCR amplification, a facile fluorescence assay of liver cancer SMMC-7721 cells was successfully realized with the detection of as low as 100 cells.

Graphical abstract: Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

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Publication details

The article was received on 09 Aug 2017, accepted on 10 Oct 2017 and first published on 10 Oct 2017


Article type: Communication
DOI: 10.1039/C7CC06218D
Citation: Chem. Commun., 2017, Advance Article
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    Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

    J. Tang, X. He, Y. Lei, H. Shi, Q. Guo, J. Liu, D. He, L. Yan and K. Wang, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC06218D

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