Issue 77, 2017

Cyclic peptide production using a macrocyclase with enhanced substrate promiscuity and relaxed recognition determinants

Abstract

Macrocyclic peptides have promising therapeutic potential but the scaling up of their chemical synthesis is challenging. The cyanobactin macrocyclase PatGmac is an efficient tool for production but is limited to substrates containing 6–11 amino acids and at least one thiazoline or proline. Here we report a new cyanobactin macrocyclase that can cyclize longer peptide substrates and those not containing proline/thiazoline and thus allows exploring a wider chemical diversity.

Graphical abstract: Cyclic peptide production using a macrocyclase with enhanced substrate promiscuity and relaxed recognition determinants

Supplementary files

Article information

Article type
Communication
Submitted
28 Jul 2017
Accepted
05 Sep 2017
First published
11 Sep 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 10656-10659

Cyclic peptide production using a macrocyclase with enhanced substrate promiscuity and relaxed recognition determinants

C. N. Alexandru-Crivac, C. Umeobika, N. Leikoski, J. Jokela, K. A. Rickaby, A. M. Grilo, P. Sjö, A. T. Plowright, M. Idress, E. Siebs, A. Nneoyi-Egbe, M. Wahlsten, K. Sivonen, M. Jaspars, L. Trembleau, D. P. Fewer and W. E. Houssen, Chem. Commun., 2017, 53, 10656 DOI: 10.1039/C7CC05913B

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