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Cyclic peptide production using a macrocyclase with enhanced substrate promiscuity and relaxed recognition determinants

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Abstract

Macrocyclic peptides have promising therapeutic potential but the scaling up of their chemical synthesis is challenging. The cyanobactin macrocyclase PatGmac is an efficient tool for production but is limited to substrates containing 6–11 amino acids and at least one thiazoline or proline. Here we report a new cyanobactin macrocyclase that can cyclize longer peptide substrates and those not containing proline/thiazoline and thus allows exploring a wider chemical diversity.

Graphical abstract: Cyclic peptide production using a macrocyclase with enhanced substrate promiscuity and relaxed recognition determinants

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Publication details

The article was received on 28 Jul 2017, accepted on 05 Sep 2017 and first published on 11 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC05913B
Citation: Chem. Commun., 2017, Advance Article
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    Cyclic peptide production using a macrocyclase with enhanced substrate promiscuity and relaxed recognition determinants

    C. N. Alexandru-Crivac, C. Umeobika, N. Leikoski, J. Jokela, K. A. Rickaby, A. M. Grilo, P. Sjö, A. T. Plowright, M. Idress, E. Siebs, A. Nneoyi-Egbe, M. Wahlsten, K. Sivonen, M. Jaspars, L. Trembleau, D. P. Fewer and W. E. Houssen, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC05913B

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