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A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

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Abstract

We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

Graphical abstract: A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

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Publication details

The article was received on 28 Jul 2017, accepted on 22 Sep 2017 and first published on 13 Nov 2017


Article type: Communication
DOI: 10.1039/C7CC05887J
Citation: Chem. Commun., 2017, Advance Article
  • Open access: Creative Commons BY license
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    A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

    S. H. Hewitt, J. Parris, R. Mailhot and S. J. Butler, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC05887J

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