A genetically encoded cyclobutene probe for labelling of live cells

K. Liu; B. Enns; B. Evans; N. Wang; X. Shang; W. Sittiwong; P. H. Dussault; J. Guo

doi:10.1039/C7CC05580C

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A genetically encoded cyclobutene probe for labelling of live cells†

K. Liu,‡^a B. Enns,‡^a B. Evans,‡^a N. Wang,^a X. Shang,^a W. Sittiwong,^b P. H. Dussault

*^a and J. Guo

*^a

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* Corresponding authors

^a Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA
E-mail: pdussault1@unl.edu, jguo4@unl.edu

^b Department of Chemistry, Thammasat University, Bangkok, Thailand

Abstract

We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels–Alder reaction with a tetrazine.

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DOI: https://doi.org/10.1039/C7CC05580C
Article type: Communication
Submitted: 18 Jul 2017
Accepted: 06 Sep 2017
First published: 06 Sep 2017

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Chem. Commun., 2017,53, 10604-10607

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A genetically encoded cyclobutene probe for labelling of live cells

K. Liu, B. Enns, B. Evans, N. Wang, X. Shang, W. Sittiwong, P. H. Dussault and J. Guo, Chem. Commun., 2017, 53, 10604 DOI: 10.1039/C7CC05580C

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Chemical Communications

A genetically encoded cyclobutene probe for labelling of live cells†

Abstract

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A genetically encoded cyclobutene probe for labelling of live cells

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