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Issue 76, 2017
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A genetically encoded cyclobutene probe for labelling of live cells

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Abstract

We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels–Alder reaction with a tetrazine.

Graphical abstract: A genetically encoded cyclobutene probe for labelling of live cells

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Publication details

The article was received on 18 Jul 2017, accepted on 06 Sep 2017 and first published on 06 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC05580C
Citation: Chem. Commun., 2017,53, 10604-10607
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    A genetically encoded cyclobutene probe for labelling of live cells

    K. Liu, B. Enns, B. Evans, N. Wang, X. Shang, W. Sittiwong, P. H. Dussault and J. Guo, Chem. Commun., 2017, 53, 10604
    DOI: 10.1039/C7CC05580C

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