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A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids

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Abstract

Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases.

Graphical abstract: A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids

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Publication details

The article was received on 18 Jul 2017, accepted on 06 Sep 2017 and first published on 06 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC05576E
Citation: Chem. Commun., 2017, Advance Article
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    A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids

    O. Kamar, S. Sun, C. Lin, W. Chung, M. Lee, Y. Liao, D. M. Kolpashchikov and M. Chuang, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC05576E

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