Issue 80, 2017

Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase

Abstract

We developed a simple and rapid method for terminal deoxynucleotidyl transferase (TdT) assay on the basis of the polymerization-directed exonuclease-assisted construction of a single quantum dot (QD)-based fluorescence resonance energy transfer (FRET) nanosensor. This method is very sensitive with a detection limit as low as 1 × 10−6 U μL−1, and it can be used for the screening of TDT inhibitors and accurate quantification of TdT activity even in 5 cancer cells.

Graphical abstract: Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase

Supplementary files

Article information

Article type
Communication
Submitted
15 Jul 2017
Accepted
14 Sep 2017
First published
14 Sep 2017

Chem. Commun., 2017,53, 11016-11019

Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase

L. Wang, M. Luo, Q. Zhang, B. Tang and C. Zhang, Chem. Commun., 2017, 53, 11016 DOI: 10.1039/C7CC05485H

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