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Issue 80, 2017
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H. pylori α1–3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides

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Abstract

Helicobacter pylori α1–3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L−1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of L-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT).

Graphical abstract: H. pylori α1–3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides

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Publication details

The article was received on 13 Jul 2017, accepted on 14 Sep 2017 and first published on 14 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC05403C
Citation: Chem. Commun., 2017,53, 11012-11015
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    H. pylori α1–3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides

    H. Yu, Y. Li, Z. Wu, L. Li, J. Zeng, C. Zhao, Y. Wu, N. Tasnima, J. Wang, H. Liu, M. R. Gadi, W. Guan, P. G. Wang and X. Chen, Chem. Commun., 2017, 53, 11012
    DOI: 10.1039/C7CC05403C

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