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Issue 68, 2017
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A DNA-assisted immunoassay for enzyme activity via a DNA-linked, activity-based probe

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Abstract

Here, we describe an immunoassay approach for the detection of enzyme activity by quantitative PCR (qPCR) or parallel DNA sequencing which relies on activity-based probes linked to barcoding DNAs. We demonstrate this technique in the detection of serine hydrolase activities using a fluorophosphonate-oligonucleotide conjugate.

Graphical abstract: A DNA-assisted immunoassay for enzyme activity via a DNA-linked, activity-based probe

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Publication details

The article was received on 06 Jul 2017, accepted on 27 Jul 2017 and first published on 01 Aug 2017


Article type: Communication
DOI: 10.1039/C7CC05236G
Citation: Chem. Commun., 2017,53, 9474-9477
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    A DNA-assisted immunoassay for enzyme activity via a DNA-linked, activity-based probe

    D. Kim, R. R. Jetson and C. J. Krusemark, Chem. Commun., 2017, 53, 9474
    DOI: 10.1039/C7CC05236G

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