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Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes

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Abstract

An on-surface isothermal helicase-dependent amplification is devised for simple, point-of-need quantification of bacterial genomes. The method relies on the enzyme-extension of a thiol-modified reverse primer anchored to indium tin oxide electrodes, which shows strikingly high thermal and storage stability. Amplification and electrochemical detection of only 10 genomes are thus performed on the same platform without thermal cycling.

Graphical abstract: Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes

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Publication details

The article was received on 03 Jul 2017, accepted on 25 Jul 2017 and first published on 26 Jul 2017


Article type: Communication
DOI: 10.1039/C7CC05128J
Citation: Chem. Commun., 2017, Advance Article
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    Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes

    S. Barreda-García, R. Miranda-Castro, N. de-los-Santos-Álvarez, A. J. Miranda-Ordieres and M. J. Lobo-Castañón, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC05128J

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