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Issue 81, 2017
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A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

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Abstract

Herein, we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference of other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λem = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 provides information about H2O2-mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells.

Graphical abstract: A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

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Publication details

The article was received on 29 Jun 2017, accepted on 10 Sep 2017 and first published on 11 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC05043G
Citation: Chem. Commun., 2017,53, 11213-11216
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    A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

    K. Naidu Bobba, M. Won, I. Shim, N. Velusamy, Z. Yang, J. Qu, J. S. Kim and S. Bhuniya, Chem. Commun., 2017, 53, 11213
    DOI: 10.1039/C7CC05043G

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