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BODIPY based Two-Photon Fluorescent Probe Validates Tyrosinase Activity in Live Cells

Abstract

Here we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking of intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference from other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λem = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 enables to provide information of H2O2 mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells.

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Publication details

The article was received on 29 Jun 2017, accepted on 10 Sep 2017 and first published on 11 Sep 2017


Article type: Communication
DOI: 10.1039/C7CC05043G
Citation: Chem. Commun., 2017, Accepted Manuscript
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    BODIPY based Two-Photon Fluorescent Probe Validates Tyrosinase Activity in Live Cells

    K. N. bobba, M. Won, I. Shim, N. Velusamy, Z. Yang, J. Qu, J. S. Kim and S. Bhuniya, Chem. Commun., 2017, Accepted Manuscript , DOI: 10.1039/C7CC05043G

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