Issue 68, 2017
From the journal:

Chemical Communications

Fluorescence-switching RNA for detection of bacterial ribosomes†

Lihao Guoab  and  Akimitsu Okamoto*ac  

* Corresponding authors

a Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan

b Department of Advanced Interdisciplinary Studies, Graduate School of Engineering, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan

c Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
E-mail: okamoto@chembio.t.u-tokyo.ac.jp

Abstract

We have developed an efficient chemical system that allows quantification of bacterial ribosomes by fluorescence-based analysis. The key component in the system is the exciton-controlled fluorescent RNA aptamer, which recognizes neomycin B. The intensity of fluorescence from such a ribosome-sensing system increased drastically in the presence of Escherichia coli.

Graphical abstract: Fluorescence-switching RNA for detection of bacterial ribosomes

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Article information

DOI
https://doi.org/10.1039/C7CC04818A
Article type
Communication
Submitted
21 Jun 2017
Accepted
17 Jul 2017
First published
18 Jul 2017

Chem. Commun., 2017,53, 9406-9409

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Fluorescence-switching RNA for detection of bacterial ribosomes

L. Guo and A. Okamoto, Chem. Commun., 2017, 53, 9406 DOI: 10.1039/C7CC04818A

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