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Issue 68, 2017
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Endonuclease IV based competitive DNA probe assay for differentiation of low-abundance point mutations by discriminating stable single-base mismatches

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Abstract

We disclosed the unique discrimination property of Endo IV toward stable single-base mismatches located at the second nucleotide 3′ to the AP site. Coupled with thermodynamic differentiation and competitive blocker strands, a highly sensitive and specific detection system was established with discrimination factors of 510–1079 for G:X mismatches and LODs of 0.003–0.005% for KRAS G12A, KRAS G12V and KRAS G12S mutations.

Graphical abstract: Endonuclease IV based competitive DNA probe assay for differentiation of low-abundance point mutations by discriminating stable single-base mismatches

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Publication details

The article was received on 21 Jun 2017, accepted on 02 Aug 2017 and first published on 02 Aug 2017


Article type: Communication
DOI: 10.1039/C7CC04816E
Citation: Chem. Commun., 2017,53, 9422-9425
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    Endonuclease IV based competitive DNA probe assay for differentiation of low-abundance point mutations by discriminating stable single-base mismatches

    J. Xu, L. Li, N. Chen, Y. She, S. Wang, N. Liu and X. Xiao, Chem. Commun., 2017, 53, 9422
    DOI: 10.1039/C7CC04816E

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