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Cell type-selective imaging and profiling of newly synthesized proteomes by using puromycin analogues

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Abstract

We have developed a versatile antibody-assisted strategy for the imaging and profiling of newly synthesized proteomes in a cell-specific manner. This strategy remained highly selective even in heterogeneous co-cultured cells, thus enabling labeling and enrichment of nascent proteomes from targeted cells without the need for physical separation.

Graphical abstract: Cell type-selective imaging and profiling of newly synthesized proteomes by using puromycin analogues

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Publication details

The article was received on 12 Jun 2017, accepted on 05 Jul 2017 and first published on 05 Jul 2017


Article type: Communication
DOI: 10.1039/C7CC04536K
Citation: Chem. Commun., 2017, Advance Article
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    Cell type-selective imaging and profiling of newly synthesized proteomes by using puromycin analogues

    S. Du, D. Wang, J. Lee, B. Peng, J. Ge and S. Q. Yao, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC04536K

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