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Engineering multi-functional bacterial outer membrane vesicles as modular nanodevices for biosensing and bioimaging

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Abstract

Outer membrane vesicles (OMVs) are proteoliposomes derived from the outer membrane and periplasmic space of many Gram-negative bacteria including E. coli as part of their natural growth cycle. Inspired by the natural ability of E. coli to sort proteins to both the exterior and interior of OMVs, we reported here a one-pot synthesis approach to engineer multi-functionalized OMV-based sensors for both antigen binding and signal generation. SlyB, a native lipoprotein, was used a fusion partner to package nanoluciferase (Nluc) within OMVs, while a previously developed INP-Scaf3 surface scaffold was fused to the Z-domain for antibody recruiting. The multi-functionalized OMVs were used for thrombin detection with a detection limit of 0.5 nM, comparable to other detection methods. Using the cohesin domains inserted between the Z-domain and INP, these engineered OMVs were further functionalized with a dockerin-tagged GFP for cancer cell imaging.

Graphical abstract: Engineering multi-functional bacterial outer membrane vesicles as modular nanodevices for biosensing and bioimaging

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Publication details

The article was received on 01 Jun 2017, accepted on 16 Jun 2017 and first published on 16 Jun 2017


Article type: Communication
DOI: 10.1039/C7CC04246A
Citation: Chem. Commun., 2017, Advance Article
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    Engineering multi-functional bacterial outer membrane vesicles as modular nanodevices for biosensing and bioimaging

    Q. Chen, S. Rozovsky and W. Chen, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC04246A

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