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An efficient target–intermediate recycling amplification strategy for ultrasensitive fluorescence assay of intracellular lead ions

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Abstract

An ultrasensitive fluorescence assay for intracellular Pb2+ determination was proposed through target–intermediate recycling amplification based on metal-assisted DNAzyme catalysis and strand displacement reactions. Compared with only target recycling-based fluorescence assay with an M amplification ratio, the proposed assay could achieve an M × N amplification ratio to obtain an improved sensitivity by more than 10 times, in which M and N are the amplification ratios of target recycling and intermediate recycling, respectively. Remarkably, this proposed ultrasensitive fluorescence assay could be applied to the determination of various analytes with the well-designed detection probe, especially in intracellular assay, providing a promising tool for clinical diagnosis and biomedical detection.

Graphical abstract: An efficient target–intermediate recycling amplification strategy for ultrasensitive fluorescence assay of intracellular lead ions

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Publication details

The article was received on 27 May 2017, accepted on 13 Jun 2017 and first published on 13 Jun 2017


Article type: Communication
DOI: 10.1039/C7CC04104G
Citation: Chem. Commun., 2017, Advance Article
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    An efficient target–intermediate recycling amplification strategy for ultrasensitive fluorescence assay of intracellular lead ions

    Z. Wen, W. Liang, Y. Zhuo, C. Xiong, Y. Zheng, R. Yuan and Y. Chai, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC04104G

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