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Issue 54, 2017
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Solventless mechanochemical preparation of novel magnetic bioconjugates

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Abstract

A solventless mechanochemical approach was employed to obtain a bioconjugate (BSA–DA–Fe2O3) based on bovine serum albumin (BSA) and dopamine (DA) coated iron oxide magnetic nanoparticles. UV-vis measurements of the obtained material showed a distinctive peak at 280 nm which, together with the presence of N on the surface of the nanomaterial (a band at 400 eV in the XPS spectrum) and zeta potential measurements, confirmed the successful immobilization of the protein. Additionally, the presence of two bands at 1652 and 1545 cm−1 in the FT-IR spectra of both BSA and BSA–DA–Fe2O3 and steady-state fluorescence analysis validated that the protein preserved its native-like structure after the mechanochemical milling process. Also the functionalized MNPs preserved their magnetic properties as have been demonstrated by their magnetic susceptibility value.

Graphical abstract: Solventless mechanochemical preparation of novel magnetic bioconjugates

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Publication details

The article was received on 23 May 2017, accepted on 15 Jun 2017 and first published on 15 Jun 2017


Article type: Communication
DOI: 10.1039/C7CC03975A
Citation: Chem. Commun., 2017,53, 7635-7637
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    Solventless mechanochemical preparation of novel magnetic bioconjugates

    D. Rodriguez-Padrón, A. R. Puente-Santiago, A. M. Balu, A. A. Romero and R. Luque, Chem. Commun., 2017, 53, 7635
    DOI: 10.1039/C7CC03975A

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