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Single antibody–antigen interactions monitored via transient ionic current recording using nanopore sensors

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Abstract

Understanding the single molecular protein–protein interaction has great significance in evaluating the affinity of a specific antibody. Herein, the interaction between single α-fetal protein (AFP) and its antibody was monitored via transient ionic current recording by using the antibody functionalized nanopore sensors. More importantly, the kinetic evaluation was performed at the single molecule level to determine the dissociation constant of this interaction. This method enables the monitoring of the kinetic antigen–antibody interaction in their heterogenetic state without any labelling. Our results provided new insights into the evaluation of the antibody's binding affinity and more into the development of immunoassays for diagnostics.

Graphical abstract: Single antibody–antigen interactions monitored via transient ionic current recording using nanopore sensors

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Publication details

The article was received on 21 May 2017, accepted on 06 Jul 2017 and first published on 06 Jul 2017


Article type: Communication
DOI: 10.1039/C7CC03927A
Citation: Chem. Commun., 2017, Advance Article
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    Single antibody–antigen interactions monitored via transient ionic current recording using nanopore sensors

    Y. Ying, R. Yu, Y. Hu, R. Gao and Y. Long, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC03927A

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