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Issue 66, 2017
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Albumin removal from human serum using surface nanopockets on silica-coated magnetic nanoparticles

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Abstract

Selective removal of albumin from human serum is an essential step prior to proteomic analyses, especially when using mass spectrometry. Here we report stable synthetic nanopockets on magnetic nanoparticle surfaces that bind to human serum albumin (HSA) with high affinity and specificity. The nanopockets are created by templating HSA on 200 nm silica-coated paramagnetic nanoparticles using polymer layers made using 4 organo-silane monomers. These monomers have amino acid-like side chains providing hydrophobic, hydrophilic and H-bonding interactions that closely mimic features of binding sites on antibodies. The binding capacity of the material was 21 mg HSA gāˆ’1, and consistently removed āˆ¼88% albumin from human serum in multiple repeated use.

Graphical abstract: Albumin removal from human serum using surface nanopockets on silica-coated magnetic nanoparticles

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Publication details

The article was received on 02 May 2017, accepted on 27 Jul 2017 and first published on 27 Jul 2017


Article type: Communication
DOI: 10.1039/C7CC03412A
Citation: Chem. Commun., 2017,53, 9254-9257
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    Albumin removal from human serum using surface nanopockets on silica-coated magnetic nanoparticles

    S. Bhakta, C. K. Dixit, I. Bist, J. Macharia, M. Shen, K. Kadimisetty, J. He, B. Dutta, S. L. Suib and J. F. Rusling, Chem. Commun., 2017, 53, 9254
    DOI: 10.1039/C7CC03412A

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