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Issue 62, 2017
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Specific fluorescence labeling of target proteins by using a ligand–4-azidophthalimide conjugate

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Abstract

We herein propose a simple affinity-labeling method using a ligand–4-azidophthalimide (AzPI) conjugate. As a proof of concept, we show that two different ligand–AzPI conjugates enabled highly specific fluorescence labeling of their individual target proteins even in crude cell lysates. This method was also applied to label endogenous target proteins inside living cells.

Graphical abstract: Specific fluorescence labeling of target proteins by using a ligand–4-azidophthalimide conjugate

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Publication details

The article was received on 27 Apr 2017, accepted on 08 Jul 2017 and first published on 10 Jul 2017


Article type: Communication
DOI: 10.1039/C7CC03252H
Citation: Chem. Commun., 2017,53, 8751-8754
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    Specific fluorescence labeling of target proteins by using a ligand–4-azidophthalimide conjugate

    K. Chiba, M. Asanuma, M. Ishikawa, Y. Hashimoto, K. Dodo, M. Sodeoka and T. Yamaguchi, Chem. Commun., 2017, 53, 8751
    DOI: 10.1039/C7CC03252H

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