Jump to main content
Jump to site search


A Co-expression Strategy to Achieve Labeling of Individual Subunits within a Dimeric Protein for Single Molecule Analysis

Abstract

A generic co-expression strategy for site-specific incorporation of a single donor-acceptor dye pair into any position in a dimeric protein, allowing single molecule FRET study of proteins previously inaccessible to this technique, such as the intrinsically disordered prion N-domain of Ure2 in the context of its globular dimeric C-domain.

Back to tab navigation

Supplementary files

Publication details

The article was accepted on 07 Jun 2017 and first published on 19 Jun 2017


Article type: Communication
DOI: 10.1039/C7CC03032K
Citation: Chem. Commun., 2017, Accepted Manuscript
  •   Request permissions

    A Co-expression Strategy to Achieve Labeling of Individual Subunits within a Dimeric Protein for Single Molecule Analysis

    F. Lou, J. Yang, S. Wu and S. Perrett, Chem. Commun., 2017, Accepted Manuscript , DOI: 10.1039/C7CC03032K

Search articles by author

Spotlight

Advertisements