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A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis

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Abstract

A generic co-expression strategy for site-specific incorporation of a single donor–acceptor dye pair into any position in a dimeric protein, allowing single molecule FRET study of proteins previously inaccessible to this technique, such as the intrinsically disordered prion N-domain of Ure2 in the context of its globular dimeric C-domain.

Graphical abstract: A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis

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Publication details

The article was received on 20 Apr 2017, accepted on 07 Jun 2017 and first published on 19 Jun 2017


Article type: Communication
DOI: 10.1039/C7CC03032K
Citation: Chem. Commun., 2017, Advance Article
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    A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis

    F. Lou, J. Yang, S. Wu and S. Perrett, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC03032K

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