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DNA-friendly Cu(II)/TEMPO-catalyzed 5-hydroxymethylcytosine-specific oxidation

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Abstract

The mixture of copper(II) perchlorate and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) mildly and specifically oxidized 5-hydroxymethylcytosine to yield 5-formylcytosine because of its allyl alcohol specificity. The genomic DNA oxidized at 5-hydroxymethylcytosine by the copper(II)/TEMPO oxidation was efficiently analyzed using various practical methods: DNA cleavage by piperidine, specific functionalization by hydrazone formation, and genome-wide high-resolution microarray.

Graphical abstract: DNA-friendly Cu(ii)/TEMPO-catalyzed 5-hydroxymethylcytosine-specific oxidation

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Publication details

The article was received on 12 Apr 2017, accepted on 05 May 2017 and first published on 05 May 2017


Article type: Communication
DOI: 10.1039/C7CC02814H
Citation: Chem. Commun., 2017, Advance Article
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    DNA-friendly Cu(II)/TEMPO-catalyzed 5-hydroxymethylcytosine-specific oxidation

    T. Matsushita, Y. Moriyama, G. Nagae, H. Aburatani and A. Okamoto, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC02814H

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