Issue 43, 2017

Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis

Abstract

We report a novel method for multisite protein conjugation by setting differently silyl-protected alkynes as conjugation handles, which can remain intact through the whole synthetic procedure and provide sequential and orthogonal conjugation. This strategy enables efficient preparation of a dual dye-labeled protein and structural analysis via an intramolecular FRET mechanism.

Graphical abstract: Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis

Supplementary files

Article information

Article type
Communication
Submitted
05 Apr 2017
Accepted
05 May 2017
First published
10 May 2017

Chem. Commun., 2017,53, 5918-5921

Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis

G. Hayashi, N. Kamo and A. Okamoto, Chem. Commun., 2017, 53, 5918 DOI: 10.1039/C7CC02612A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements