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Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis

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Abstract

We report a novel method for multisite protein conjugation by setting differently silyl-protected alkynes as conjugation handles, which can remain intact through the whole synthetic procedure and provide sequential and orthogonal conjugation. This strategy enables efficient preparation of a dual dye-labeled protein and structural analysis via an intramolecular FRET mechanism.

Graphical abstract: Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis

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Publication details

The article was received on 05 Apr 2017, accepted on 05 May 2017 and first published on 10 May 2017


Article type: Communication
DOI: 10.1039/C7CC02612A
Citation: Chem. Commun., 2017, Advance Article
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    Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis

    G. Hayashi, N. Kamo and A. Okamoto, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC02612A

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