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Issue 43, 2017
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Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal

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Abstract

We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchrotron infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electrochemical reduction from the oxidised inactive to the fully reduced state, and temporally resolve steps during this reduction.

Graphical abstract: Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal

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Publication details

The article was received on 04 Apr 2017, accepted on 09 May 2017 and first published on 09 May 2017


Article type: Communication
DOI: 10.1039/C7CC02591B
Citation: Chem. Commun., 2017,53, 5858-5861
  • Open access: Creative Commons BY license
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    Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal

    P. A. Ash, S. B. Carr, H. A. Reeve, A. Skorupskaitė, J. S. Rowbotham, R. Shutt, M. D. Frogley, R. M. Evans, G. Cinque, F. A. Armstrong and K. A. Vincent, Chem. Commun., 2017, 53, 5858
    DOI: 10.1039/C7CC02591B

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