Jump to main content
Jump to site search


Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal

Author affiliations

Abstract

We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchrotron infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electrochemical reduction from the oxidised inactive to the fully reduced state, and temporally resolve steps during this reduction.

Graphical abstract: Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 04 Apr 2017, accepted on 09 May 2017 and first published on 09 May 2017


Article type: Communication
DOI: 10.1039/C7CC02591B
Citation: Chem. Commun., 2017, Advance Article
  •   Request permissions

    Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal

    P. A. Ash, S. B. Carr, H. A. Reeve, A. Skorupskaitė, J. S. Rowbotham, R. Shutt, M. D. Frogley, R. M. Evans, G. Cinque, F. A. Armstrong and K. A. Vincent, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC02591B

Search articles by author