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Issue 45, 2017
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Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore

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Abstract

Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630–640 nm lasers and displays two distinct emission bands depending on the calcium concentration (Kd = ∼8 μM). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells.

Graphical abstract: Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore

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Publication details

The article was received on 29 Mar 2017, accepted on 15 May 2017 and first published on 15 May 2017


Article type: Communication
DOI: 10.1039/C7CC02418E
Citation: Chem. Commun., 2017,53, 6117-6120
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    Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore

    M. Collot, F. Ponsot and A. S. Klymchenko, Chem. Commun., 2017, 53, 6117
    DOI: 10.1039/C7CC02418E

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