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Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

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Abstract

We expand photochromic super-resolution optical fluctuation imaging (pcSOFI) to monochromatic dual-channel sub-diffraction microscopy. Multi-tau (mt-)pcSOFI unmixes spectrally identical reversibly switchable fluorescent proteins (RSFPs) based on their blinking kinetics. We show that mt-pcSOFI can be used to simultaneously image two structures in living cells with existing RSFPs and the newly developed ffDronpa-F.

Graphical abstract: Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

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Publication details

The article was received on 27 Mar 2017, accepted on 19 May 2017 and first published on 31 May 2017


Article type: Communication
DOI: 10.1039/C7CC02344H
Citation: Chem. Commun., 2017, Advance Article
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    Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

    S. Duwé, W. Vandenberg and P. Dedecker, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC02344H

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