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Issue 43, 2017
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Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method

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Abstract

Herein, we report a kisscomplex based protein fluorescence assay (KPFA) method, which employed the formation of a kisscomplex and the T7 RNA polymerase amplification method, for the assay of transcription factors with high sensitivity.

Graphical abstract: Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method

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Publication details

The article was received on 24 Mar 2017, accepted on 09 May 2017 and first published on 09 May 2017


Article type: Communication
DOI: 10.1039/C7CC02231J
Citation: Chem. Commun., 2017,53, 5846-5849
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    Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method

    K. Zhang, K. Wang, X. Zhu and M. Xie, Chem. Commun., 2017, 53, 5846
    DOI: 10.1039/C7CC02231J

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