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Issue 35, 2017
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Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

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Abstract

There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid–liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.

Graphical abstract: Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

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Publication details

The article was received on 17 Feb 2017, accepted on 23 Mar 2017 and first published on 23 Mar 2017


Article type: Communication
DOI: 10.1039/C7CC01289F
Citation: Chem. Commun., 2017,53, 4775-4778
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    Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

    M. Sobrinos-Sanguino, S. Zorrilla, C. D. Keating, B. Monterroso and G. Rivas, Chem. Commun., 2017, 53, 4775
    DOI: 10.1039/C7CC01289F

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